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KMID : 0365819730130010109
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Journal of Pusan Medical College
1973 Volume.13 No. 1 p.109 ~ p.115
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Immunoserological Studies on the Antimicrococcus lysodeikticus Rabbit and Chicken Serums
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Abstract
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Antimicroco_cus lysodeikticus serums were obtaind by rabbits and chicken immunized with killed Micrococcus lysodeikticus suspension at weekly intervals for 5 weeks. Seven days after the last injection the experimental animals were bled. and the antimicrococcus lysodeikticus serums were tested for the following immunoserolog ical reactions.
The author studied bacterial agglutination, passive hemagglutination,and modified hemolytic reactions with antimicrococcus lysodeikticus serums, and blocking test for bacteriolysis reaction to lysozyme. and compared tanned, trypsinzed, and bis-diazotized henzidine (BDB) treated erythrocytes on hemagglutination reactions, and modified hemolytic reactions with non-treated erythrocytes, The results obtained were as follows
1. The titer of antimicrococcus lysodeikticus rabbit serum was higher than antimicrococcus lysokticus chicken serum on the bacterial agglutination.
2. Enzymatic activity of lysozyme bacteriolysis reaction was blocked by the antimicrococcus lysodelkticus serum. The blocking activity of chicken antiserum was higher than that of rabbit antiserum.
3. Tanned erythrocytes was more sensitive than trypsinized and BDB treated erythrocytes on the passive hemagglutination and hemolytic reaction in the antimicrococcus lysodeikticus rabbit serum. But tanned erythrocytes, except trypsinized and BDB treated erythrocytes, was not suitable for test of passive hemagglutination and hemolytic reaction due to non-specific hernagglutination in the antimicrococcus lysodeikticus chicken serum.
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